Applied Spectroscopy November Highlights
This month's Focal Point was written by an Applied Spectroscopy Associate Editor, Professor Meiping Zhao from College of Chemistry and Molecular Engineering at Peking University, China. Zhao's research work focuses on development of fluorescent biosensors based on DNA-enzyme interactions, molecular imprinting techniques and microfluidic electrophoresis. Her group designs novel fluorescent probes for detection of low-abundance DNA mutations, measurement of MiRNA variations at single-cell level, fluorescence imaging of enzymes in living cells and real-time monitoring of hormones and neuropeptides.
The article focuses on recent developments in biosensing methods based on three types of nucleic acid fluorescent probes, including hybridization probes, affinity probes (aptamers) and enzymatic substrate probes. Different fluorescence detection techniques for these probes are reviewed, including fluorescence imaging approaches. The article concludes with a perspective on future directions for the development of nucleic acid fluorescent probes.
See Applied Spectroscopy, Volume 66, Number 11 (2012) for a full list of articles.