holder

ATTENTION: This site is down for maintenance in read-only mode.

The following is an abstract for the selected article. A PDF download of the full text of this article is available here. Members may download full texts at no charge. Non-members may be charged a small fee for certain articles.


Low-Frequency Spectroscopic Analysis of Monomeric and Fibrillar Lysozyme

Volume 65, Number 3 (March 2011) Page 260-264

HIDAYATUL A. ZAKARIA, BERND M. FISCHER, ANDREW P. BRADLEY, INKE JONES, DEREK ABBOTT, ANTON P. J. MIDDELBERG, and ROBERT J. FALCONER*


Terahertz time-domain spectroscopy (THz-TDS) and Fourier transform infrared (FT-IR) spectroscopy were used to generate far-infrared and low-frequency spectral measurements of monomeric lysozyme and lysozyme fibrils. The formation of lysozyme fibrils was verified by the Thioflavin T assay and transmission electron microscopy (TEM). It was evident in the FT-IR spectra that between 150 and 350 cm-1 the two spectra diverge, with the lysozyme fibrils showing higher absorbance intensity than the monomeric form. The broad absorption phenomenon is likely due to light scattered from the fibrillar architecture of lysozyme fibrils as supported by simulation of Rayleigh light scattering. The lack of discrete phonon-like peaks suggest that far-infrared spectroscopy cannot detect vibrational modes between the highly ordered hydrogen-bonded beta-pleated sheets of the lysozyme subunit.

Index Headings: Terahertz; Time-domain spectroscopy; THz-TDS; Fourier transform infrared spectroscopy; FT-IR spectroscopy; Vibrational spectroscopy; Lysozyme; Amyloid; Fibril.