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A 5-kg Time-Resolved Luminescence Photometer with Multiple Excitation Sources

Volume 66, Number 3 (March 2011) Page 341-346

GUOYING CHEN


A luminescence photometer was developed based on lanthanide-sensitized luminescence to detect environmental pollutants and residues in foods including, in particular, two classes of antibiotics: tetracyclines and fluoroquinolones. Multiple excitation sources, a xenon flashlamp and ultraviolet light-emitting diodes (UV LEDs), were used to their advantages. A photomultiplier tube module, gated to reject time-domain interferences, was used as a photodetector. Using danofloxacin as a model analyte, luminescence signal was linear in more than three decades (0.5-2000 ng/mL) with R2>0.998 in each decade. This photometer achieved a limit of detection of 2.0 ng/mL with 1.3% average relative standard deviation. It is field deployable at 4.6 kg and 15 W power consumption.

Index Headings: Photometer; Lanthanide-sensitized luminescence; Fluoroquinolone; Tetracycline; LED; Xenon flashlamp.