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Selection of Haploid Maize Kernels from Hybrid Kernels for Plant Breeding Using Near-Infrared Spectroscopy and SIMCA Analysis

Volume 66, Number 4 (April 2012) Page 447-450

ROGER W. JONES,* TONU REINOT, URSULA K. FREI, YICHIA TSENG, THOMAS LÜBBERSTEDT, and JOHN F. MCCLELLAND


Samples of haploid and hybrid seed from three different maize donor genotypes after maternal haploid induction were used to test the capability of automated near-infrared transmission spectroscopy to individually differentiate haploid from hybrid seeds. Using a two-step chemometric analysis in which the seeds were first classified according to genotype and then the haploid or hybrid status was determined proved to be the most successful approach. This approach allowed 11 of 13 haploid and 25 of 25 hybrid kernels to be correctly identified from a mixture that included seeds of all the genotypes.



Index Headings: Near-infrared spectroscopy; NIR spectroscopy; Corn; Maize; Haploid selection; Partial least squares; PLS; Single kernel analysis; Soft independent modeling of class analogy; SIMCA.