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Differentiation of Neotropical Fish Species with Statistical Analysis of Fourier Transform Infrared Photoacoustic Spectroscopy Data

Volume 66, Number 7 (July 2012) Page 782-785

FRANCYLAINE S. ALMEIDA, SANDRO M. LIMA, LUIS H.C. ANDRADE, and YZEL R. SÚAREZ*


Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) was applied to nineteen fish species in Brazil's Upper Paraná River basin to identify differences in the structural composition of their scales. To differentiate the species, a canonical discriminant analysis was used to indicate the most important absorption peaks in the mid-infrared region. Significant differences were found in the chemical composition of scales among the studied fish species, with Wilk's lambda = 5.2 × 10−6, F(13,18,394) = 37.57, and P < 0.001, indicating that O–CH2 wag at 1396 cm−1 can be used as a biomarker of this species group. The species could be categorized into four groups according to phylogenetic similarity, suggesting that the O–CH2 1396 cm−1 absorbance is related to the biological traits of each species. This procedure can also be used to complement evolutionary studies.



Index Headings: Fourier transform infrared photoacoustic spectroscopy; FT-IR-PAS; Neotropical fish biomarker; Canonical discriminant analysis.