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Infrared Spectral Properties of Germ, Pericarp, and Endosperm Sections of Sound Wheat Kernels and Those Damaged by Fusarium graminearum

Volume 66, Number 9 (Sept. 2012) Page 1053-1060

KAMARANGA H. S. PEIRIS, WILLIAM W. BOCKUS, and FLOYD E. DOWELL*


Mid-infrared attenuated total reflection (Mid-IR-ATR) spectra (4000–380 cm−1) of pericarp, germ, and endosperm sections from sound and Fusarium-damaged wheat kernels of cultivars Everest and Tomahawk were collected using a Fourier transform infrared (FT-IR) spectrometer. The differences in infrared absorption bands between sound and Fusarium-damaged kernels were examined. Absorption bands in which differences were identified were compared with the mid-IR-ATR absorption bands of deoxynivalenol (DON) and Fusarium graminearum hyphae. Marked differences in absorption patterns were observed between sound and Fusarium-damaged pericarp and germ spectra, whereas those differences were negligible in the endosperm spectra. Fusarium-damaged pericarp and germ spectra exhibited a shift in the peak position of the band near 1035 cm−1 along with increased absorptions at 1160, 1203, 1313, and 1375 cm−1, likely due to the influence of DON and fungi in the Fusarium-damaged kernel tissue matrix. These results suggest that infrared spectroscopy can detect DON in the surface tissues of Fusarium-damaged wheat kernels.



Index Headings: Wheat; Fusarium graminearum; Fusarium head blight; Deoxynivalenol; DON; Attenuated total reflection spectroscopy; ATR spectroscopy; Fourier transform infrared spectroscopy; FT-IR spectroscopy.