The following is an abstract for the selected article. A PDF download of the full text of this article is available here. Members may download full texts at no charge. Non-members may be charged a small fee for certain articles.
Facile Characterization of the Immobilization of Streptavidin on Magnetic Submicron Particles with a Fluorescent Probe of Streptavidin
Volume 67, Number 6 (June 2013) Page 688-691
GAOBO LONG, YI ZHANG, XIAOLAN YANG, JUN PU, JIALIN QIN, LIN LIU, and FEI LIAO*
To characterize streptavidin immobilization on magnetic submicron particles (MSPs), residual streptavidin after magnetic removal of immobilized streptavidin was quantified with N-biotinyl-N′-(1-naphthyl)-ethylenediamine (BNEDA) based on Förster resonance energy transfer. Residual BNEDA after magnetic removal of bound BNEDA was measured by its own fluorescence. Streptavidin was immobilized at about 12 mg per gram of MSPs and easily retained over 50% of its original activity. These assays facilitated optimized streptavidin immobilization on MSPs.
Index Headings: Magnetic submicron particles; N-biotinyl-N′-(1-naphthyl)-ethylenediamine; Fluorometric assays; Streptavidin.