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Resonance Raman Evidence of Immobilization of Laccase on Self-Assembled Monolayers of Thiols on Ag and Au Surfaces

Volume 60, Number 7 (July 2006) Page 752-757

Michota-Kaminska, A.; Wrzosek, B.; Bukowska, J.


Resonance Raman (RR) and surface-enhanced Raman scattering (SERS) spectroscopy have been used to study immobilization of laccase on self-assembled monolayers (SAMs) of thiols containing carboxylic and amino groups, deposited on silver and gold electrodes. A new, indirect way of monitoring laccase bound to the thiol-coated Ag and Au surfaces is presented. It was demonstrated that by recording the resonance Raman spectra of the colored product of the oxidation of syringaldazine (4-hydroxy-3,5-dimethoxybenzaldehydeazine) by laccase in the presence of molecular oxygen, one may easily confirm binding as well as enzymatic activity of laccase immobilized on the SAMs modified silver and gold surfaces.