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Temperature-Jump Apparatus with Raman Detection Based on a Solid-State Tunable (1.802.05 m) kHz Optical Parametric Oscillator Laser
Volume 60, Number 4 (April 2006) Page 347-351
Balakrishnan, Gurusamy; Hu, Ying; Spiro, Thomas G.
The operating characteristics of a pulsed (10 ns) tunable near-infrared (NIR) laser source are described for temperature-jump (T-jump) applications. A Q-switched Nd:YLF laser (∼10 ns pulses) with a 1 kHz repetition rate is used to pump a potassium titanyl arsenate (KTA) crystal-based optical parametric oscillator (OPO), producing ∼1 mJ NIR pulses that are tunable (1.80-2.05 μm) across the 1.9 μm vibrational overtone band of water. This T-jump source has been coupled to a deep ultraviolet (UV) probe laser for Raman studies of protein dynamics. T-jumps of up to 30 °C, as measured via the O-H stretching Raman band of water, are readily achieved. Application to cytochrome c unfolding is demonstrated.