The following is an abstract for the selected article. A PDF download of the full text of this article is available here. Members may download full texts at no charge. Non-members may be charged a small fee for certain articles.

The Rapid Identification of Bacteria Using Time-Resolved Fluorescence and Fluorescence Excitation Spectral Methods

Volume 39, Number 5 (Oct. 1985) Page 869-872

Brahma, S.K.; Baek, M.P.; Gaskill, D.; Force, R.K.; Nelson, W.H.; Sperry, J.

Time-resolved fluorescence spectra have been obtained for Escherichia coli, Pseudomonas fluorescens, Staphylococcus epidermidis, and Enterobacter cloacae. Pseudomonas fluorescens has been shown to have distinctly different time-resolved spectra. Fluorescence excitation spectra for the three other organisms showing similar time-resolved spectra are very nearly alike, while spectra of Pseudomonas fluorescens are markedly different. This suggests that the changes in the average fluorescence lifetime with emission wavelength are due to the separate contributions from fluorophores of distinctly different lifetimes which have emission maxima at different wavelengths.