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A Programmed Cell Instrument for Digital Raman Difference Spectroscopy

Volume 28, Number 3 (June 1974) Page 262-269

Amy, Jon W.; Chrisman, Ray W.; Lundeen, James W.; Ridley, Thomas Y.; Sprowles, Jolyon C.; Tobias, R. Stuart


An instrument for digital Raman difference spectroscopy which uses a programmed sample carrier for automated alternate positioning of reference and sample cells in the laser beam is described. Conventional single photon counting equipment is employed with its attendant advantages over dc analog methods. Signal processing is done with a digital computer. There are relatively few limitations on the types of sample cells which may be used. Applications to studies of dilute aqueous solutions of biological molecules, particularly, are discussed.