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Antibody-Based Fiberoptics Biosensor for the Carcinogen Benzo(a)pyrene

Volume 41, Number 5 (July 1987) Page 735-738

Vo-Dinh, T.; Tromberg, B.J.; Griffin, G.D.; Ambrose, K.R.; Sepaniak, M.J.; Gardenhire, E.M.


A new antibody-based fiberoptics biosensor was used to detect the important carcinogen benzo(a)pyrene (BaP). The fiberoptics sensor utilizes anti-BaP antibodies covalently bound to its tip. A helium cadmium laser was used as the excitation source to induce fluorescence from BaP conjugated to the bound anti-BaP antibodies. The fiberoptics device can detect 1 femtomole of BaP in a 5-μL sample drop.