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Utilizing Three-Dimensional Fluorescence's Red-Shift Cascade Effect to Monitor Mycobacterium PRY-1 Degradation of Aged Petroleum

Volume 58, Number 1 (Jan. 2004) Page 91-95

Sinski, Joseph F.; Compton, Bradley S.; Perkins, B. Shea; Nicoson, Michael C.


Samples of Mycobacterium PRY-1 inoculated motor oil are subjected to three-dimensional fluorescence spectroscopy to document the shifting of excitation/emission maxima as the solutions undergo serial dilutions. Effects such as self-quenching of individual polycyclic aromatic hydrocarbons (PAHs) and energy transfer between PAHs combine to produce large red-shifts in the resulting fluorescence emission spectra. This process is repeated over a series of weeks and is compared to preceding spectra to gauge the microbial degradation of the petroleum. Results show a two-fold, or 75%, PAH contaminant degradation by Mycobacterium PRY-1 over a 140-day growth period.