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Sensitivity Calibration of Multichannel Raman Spectrometers Using the Least-Squares-Fitted Fluorescence Spectrum of Quinine

Volume 42, Number 1 (Jan. 1988) Page 12-14

Iwata, K.; Hamaguchi, H.; Tasumi, M.


A method of sensitivity calibration of multichannel Raman spectrometers is described which employs the fluorescence spectrum of quinine as an intensity standard. The reported absolute fluorescence spectrum of quinine was least-squares fitted with a polynomial so that it gave an interpolation of the spectrum in the "Raman scale." The obtained least-squares-fitted fluorescence spectrum was cross-checked with the rotational Raman spectrum of D2. The fluorescence intensities calculated for every 100 cm−1 from the two Ar+ laser lines are tabulated for convenience.