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Photodeposition of Silver Can Result in Metal-Enhanced Fluorescence

Volume 57, Number 5 (May 2003) Page 526-531

Geddes, Chris D.; Parfenov, Alexandr; Lakowicz, Joseph R.


Chemically deposited silver particles are widely used for surface-enhanced Raman scattering (SERS) and more recently for surface-enhanced fluorescence (SEF), also known as metal-enhanced fluorescence (MEF). We now show that metallic silver deposited by laser illumination results in an ~7-fold increased intensity of locally bound indocyanine green. The increased intensity is accompanied by a decreased lifetime and increased photostability. These results demonstrate the possibility of photolithographic preparation of surfaces for enhanced fluorescence in microfluidics, medical diagnostics, and other applications.