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High-Precision Fluorimetry with a Light-Emitting Diode Source

Volume 42, Number 8 (Dec. 1988) Page 1469-1472

Smith, B.W.; Jones, B.T.; Winefordner, J.D.


A simple fluorimeter which uses a light-emitting diode as a source of excitation and a photodiode as a detector has been constructed and evaluated. The exceptional stability of both the detector and the source make it possible to make concentration measurements by fluorimetry with a precision of at least 0.002%. Furthermore, the system exhibited excellent linear dynamic range and a limit of detection of 20 ng/L for the dye Oxazine 720 in methanol.