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Component Spectrum Reconstruction from Partially Characterized Mixtures

Volume 43, Number 1 (Jan. 1989) Page 123-128

Nyden, Marc R.; Chittur, Krishnan

A mathematical analysis of some existing approaches to component spectrum reconstruction is presented. This analysis leads to the derivation of a generalization of the cross-correlation technique. The effectiveness of these methods is assessed from the quality of the reconstructions obtained with the use of synthetic mixture spectra. Reconstructions of the spectra of the components of aqueous mixtures of immunoglobulin G and albumin are compared to the corresponding spectral reconstructions of the pure proteins in buffer.