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Phase-Resolved Fiber-Optics Fluoroimmunosensor

Volume 44, Number 1 (Jan. 1990) Page 128-132

Vo-Dinh, T.; Nolan, T.; Cheng, Y.F.; Sepaniak, M.J.; Alarie, J.P.

This paper describes the development and application of a phase-resolved fiber-optics fluoroimmunosensor (PR-FIS), which combines the biochemical specificity of antigen-antibody reaction, the high sensitivity of laser excitation, the versatility of fiber-optics sensors, and the selectivity of phase-resolved detection. The PR-FIS device can differentiate benzo(a)pyrene and benzo(a)pyrene tetrol, a DNA-adduct product of the carcinogen benzo(a)pyrene. Benzo(a)pyrene tetrol can be detected at femtomole levels in the presence of an amount of interfacing benzo(a)pyrene that is 50-fold more concentrated.