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Resonance Raman and UV-Visible Spectroscopic Studies of Water-Soluble Sapphyrin Derivative: Drug Localization in Tumor and Normal Mice Tissues

Volume 55, Number 2 (Feb. 2001) Page 142-148

Synytsya, Alla; Král, Vladimír; Poučková, Pavla; Volka, Karel

Resonance Raman (RR) and UV-visible (UV-vis) spectroscopy have been applied to the study of sapphyrin localization in tumor human pancreatic adenocarcinoma-bearing mice. The photosensitizer was subcutaneously injected, and the kinetics of drug biodistribution have been followed in a time scale from 1 to 9 days. RR spectra of tumor and tissue samples and UV-vis absorption spectra of tissue extracts in water/methanol (1:4 v/v) mixture were measured. Spectral data were used to identify the presence and to estimate the relative content of sapphyrin in various tissues at various times after the last injection. Results showed a preferential localization of sapphyrin in tumor tissue. Maximal content of drug in tumor tissue was observed at 6 days after the last injection. Results obtained by RR and UV-vis spectroscopy were in good agreement with each other.