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Design and Applications of Rapid-Scan Spectrally Resolved Fluorescence Microscopy

Volume 54, Number 12 (Dec. 2000) Page 1727-1733

Haralampus-Grynaviski, Nicole M.; Stimson, Michael J.; Simon, John D.

This paper addresses the design and applications of a spectrally resolved confocal fluorescence microscope that measures spectral information for each pixel in the image with a fast multi-point approach. The combined spatial and spectral resolution of the device is demonstrated by performing two-photon imaging of mixtures of fluorescently labeled microspheres. Overlapping emission spectra of dye solutions contained in microcapillaries are readily distinguished. Images of a human skin tissue section and a mouse kidney section are presented which demonstrate that the structure and spectra of biologic samples can be resolved.