The following is an abstract for the selected article. A PDF download of the full text of this article is available here. Members may download full texts at no charge. Non-members may be charged a small fee for certain articles.
Rapid Detection of Bacterial Growth by Infrared Spectrophotometry
Volume 45, Number 8 (Oct. 1991) Page 1366-1367
Iqbal, Mohammad S.; Rashid, Farooq
A rapid and sensitive method based on measurement of carbon dioxide released from a culture medium as a result of bacterial metabolism of glucose is reported. The rate of release of carbon dioxide was measured by recording the absorbance at 2353 cm−1 (4.25 μm) against time using a standard infrared spectrophotometer. Detection of growth from only five organisms of Salmonella typhosa was significant after just fifteen minutes. In about three hours a clear pattern of growth was obtained by this method. The results are reproducible with a standard deviation around ±0.5%.