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Rapid Detection of Bacterial Growth by Infrared Spectrophotometry

Volume 45, Number 8 (Oct. 1991) Page 1366-1367

Iqbal, Mohammad S.; Rashid, Farooq


A rapid and sensitive method based on measurement of carbon dioxide released from a culture medium as a result of bacterial metabolism of glucose is reported. The rate of release of carbon dioxide was measured by recording the absorbance at 2353 cm−1 (4.25 μm) against time using a standard infrared spectrophotometer. Detection of growth from only five organisms of Salmonella typhosa was significant after just fifteen minutes. In about three hours a clear pattern of growth was obtained by this method. The results are reproducible with a standard deviation around ±0.5%.