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Optical Absorption and Fluorescence Spectral Imaging Using Fiber Bundle Image Compression

Volume 53, Number 9 (Sept. 1999) Page 1118-1122

McClain, Brian L.; Jiaying,; Ben-Amotz, Dor


Optical absorption and fluorescence spectral imaging is performed by using the recently developed fiber-bundle image compression (FIC) technique in which an entire spectral image is collected in a single scan of a charged-coupled device (CCD) detector. Absorption imaging is demonstrated by mapping the optical absorbance of a stained microscopic lily plant stem section. Fluorescence imaging is demonstrated by mapping shifts in the ruby R1 fluorescence line to determine the pressure distribution in a microcrystalline ruby powder squeezed between two diamond surfaces. The advantages and limitations of the FIC method, relative to tunable filter and line imaging techniques, are discussed.