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Direct Qualitative and Quantitative Analysis of Triterpenes Using 13C NMR Spectroscopy Exemplified by Dichloromethanic Extracts of Cork

Volume 53, Number 3 (March 1999) Page 344-350

Castola, Vincent; Bighelli, Ange; Casanova, Joseph


We describe a methodology which allows identification and quantitation of individual triterpenes in natural mixtures, using the computer-aided analysis of their 13C NMR spectra, without previous separation. The analytical procedure was validated on artificial mixtures and applied to authentic extracts of cork. Identification of triterpenes was possible with the use of either reference spectra recorded in our laboratory or literature data.