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Third-Harmonic Generation and Multi-Photon Excitation Fluorescence Imaging Microscopy Techniques for Online Art Conservation Diagnosis

Volume 63, Number 3 (March 2009) Page 280-285

Gualda, Emilio J.; Filippidis, George; Melessanaki, Kristalia; Fotakis, Costas


We present an appropriate methodology and results for using third-harmonic generation (THG) modality for nondestructive high resolution imaging measurements of varnished structures in model painted artifacts. Detection takes place in the reflection mode, demonstrating the ability of the technique to be applied to the evaluation of original artworks. Furthermore, multi-photon excitation fluorescence images were obtained, providing complementary information related to the identification of the chemical composition of the artifacts.