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Interaction of Sarcoplasmic Reticulum Membranes with Myotoxin A Isolated from Crotalus viridis viridis Venom Studied by Raman Spectroscopy

Volume 47, Number 6 (June 1993) Page 852-854

Liddle, Kevin; Tu, Anthony T.

Myotoxin a is a muscle-degenerating toxin isolated from the venom of Crotalus viridis viridis (prairie rattlesnake). It is composed of 42 amino acid residues with four disulfide bonds. The presence of myotoxin a and related myotoxins is common in various snake venoms. It was found recently that myotoxin a is an uncoupler of calcium ion influx in sarcoplasmic reticulum (SR). Myotoxin a is known to interact with artificial membranes. Since the biological action of myotoxin a affects the calcium pump that is present in the SR membranes, it is of interest to investigate the interaction of SR membrane with myotoxin a by Raman spectroscopy. Raman spectroscopy is particularly useful in membrane study because the C-C and C-H stretching vibrational bands are particularly sensitive in phospholipid phase transition from gel to liquid crystal.