The following is an abstract for the selected article. A PDF download of the full text of this article is available here. Members may download full texts at no charge. Non-members may be charged a small fee for certain articles.
Fluorescent Enzymatic Probes Suitable for Two-Photon Excitation
Volume 52, Number 2 (Feb. 1998) Page 298-302
Overway, Kenneth S.; Lytle, Fred E.
Several common aminopeptidase probes are examined for the strength of two-photon excited fluorescence. The fluorophores 4- methoxy- beta -naphthylamine and 7-amino-4-methylcoumarin have limits of detection of 930 pM and 200 pM, respectively. The coumarin derivative is shown to have a two-photon cross section at 590 nm, which is exceptionally large for an aromatic amine. Spectral data for this compound also indicate that excitation near 690 nm should permit quantitation at concentrations as low as 40 pM.