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Effects of Water Loading on the Fluorescein/Anti-fluorescein Antibody System in Aerosol-OT Reverse Micelles Formed in n -Heptane

Volume 52, Number 1 (Jan. 1998) Page 96-100

Lundgren, Jeffrey S.; Watkins, A. Neal; Bright, Frank V.


We report on the behavior of monoclonal 4-4-20 mouse anti-fluorescein antibodies sequestered within aerosol-OT (AOT) reverse micelles formed in liquid n -heptane as a function of water loading ( R ). Our data suggest that the fluorescein binding site structure is unaffected by R. However, we note that there is a region, at intermediate R values, where the fluorescein apparently binds even more strongly to the monoclonal anti-fluorescein antibodies compared to the native antibody in solution. These results are qualitatively explained in terms of the Ruckenstein-Karpe model, which is based on charge disproportionation and redistribution within the reverse micelle. The results demonstrate that R, within an AOT reverse micelle, influences the apparent anti-fluorescein affinity.