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Utilization of a Prism Monochromator as a Sharp-Cut Bandpass Filter in Ultraviolet Raman Spectroscopy

Volume 47, Number 8 (Aug. 1993) Page 1283-1285

Hashimoto, Shinji; Ikeda, Takeshi; Takeuchi, Hideo; Harada, Issei

Ultraviolet resonance Raman (UVRR) spectroscopy is a useful technique for studying the structure and dynamics of biological molecules. Aromatic amino acid side chains in proteins and nucleic acid bases in DNA are examples of UV chromophores in biological systems, and the Raman scattering from such chromophores is resonance-enhanced with UV excitation. By tuning the UV excitation wavelength, one can observe the Raman scattering from a particular chromophore selectively and obtain structural information that is otherwise buried in the spectra. Another advantage of UVRR spectroscopy is that fluorescence from impurities in the sample or the sample itself appears in much longer wavelength regions than the Raman scattering, and the Raman spectrum becomes virtually fluorescence free. With these merits, UVRR spectroscopy has been increasingly applied to the structural studies of biomolecules and biomolecular assemblies.