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Line-Scan Raman Microspectrometry for Biological Applications

Volume 51, Number 11 (Nov. 1997) Page 1607-1612

Grauw, C. J. De; Otto, C.; Greve, J.

A high-resolution confocal line-scan Raman microscope was developed for the study of biological samples such as cells and chromosomes. With the illumination of a line on the sample, all the spectral information of the line is recorded. The line-scan microscope was attained by the introduction of one scanning mirror, a slit, and two cylindrical lenses in a standard confocal Raman microscope. The resolution, for an ideal sample, is 0.5 mu m in the lateral direction and 3.5 mu m in the axial direction. Line-scan experiments were performed to demonstrate the applicability of the instrument for biological samples. Line-scan images of calcium phosphate bone implant coatings and of the banding pattern of polytene chromosomes are presented.