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Application of Monte Carlo Integration to Three-Dimensional Fluorescence Spectrometry

Volume 49, Number 9 (Sept. 1995) Page 1239-1242

Yan, Yuan; Xu, Jin-Gou; Lin, Zhu-Guang; Zhao, Yi-Bin; Chen, Guo-Zhen


In this paper, the sensitivity of three-dimensional fluorescence spectrometry (TDFS) is studied by using the total fluorescence intensity, calculated by Monte Carlo integration, instead of conventional single-point intensity. It has been proven that the sensitivity of the total fluorescence intensity method is nearly one hundred times better than that of the single-point intensity method for the determinations of fluorescein and tryptophan. The mechanism of the method has also been studied.