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Time-Dependent Fluorescence Depolarization Analysis in Three-Dimensional Microspectroscopy

Volume 49, Number 2 (Feb. 1995) Page 224-228

Koshioka, Masanori; Sasaki, Keiji; Masuhara, Hiroshi


Three-dimensional space- and time-resolved fluorescence depolarization spectroscopy has been developed for elucidating picosecond rotational relaxation processes occurring in micrometer-sized volumes. Anisotropy decays are obtained by a new analytical method which is adapted to the large-solid-angle characteristic of the microscope optics. System parameters introduced in this method were determined by measuring a reference sample. The fluorescence dynamics of p-terphenyl in a thin liquid layer was analyzed as a demonstration experiment.