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Fluorescence Line-Narrowing Spectroscopy as an Off-Line Identification Method for Narrow-Bore Column Liquid Chromatography
Volume 48, Number 7 (July 1994) Page 788-795
van de Nesse, R.J.; Vinkenburg, I.H.; Jonker, R.H.J.; Hoornweg, G.P.h.; Gooijer, C.; Brinkman, U.A.T.h.; Velthorst, N.H.
Storage of the effluent from a column liquid chromatograph (LC) on a thin-layer chromatography (TLC) plate was utilized to identify analytes with fluorimetric techniques in the absence of time constraints. As an example, the deposited LC trace of an impure 1-chloropyrene sample was studied in situ by conventional excitation/emission spectroscopy and by fluorescence line-narrowing (FLN) spectroscopy, a high-resolution laser-induced fluorescence technique requiring cryogenic conditions. Pyrene and several mono-, di-, and trichlorinated pyrenes, present in the 1-chloropyrene sample, were identified with the use of the fingerprint structure of the vibrationally resolved FLN spectra. Quantitation on the basis of the FLN signals was achieved by standard addition; compounds could be detected down to the low picogram level. Various TLC materials were used to study the influence of the matrix on the FLN spectra; although peak intensities and hole burning effects are matrix-dependent, the line positions are not influenced.