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Effect of Sample and Incident Beam Areas on Quantitative Spectroscopy

Volume 48, Number 7 (July 1994) Page 891-893

Free, Michael L.; Miller, Jan D.


Quantitative spectroscopy is based upon the Bouguer-Lambert-Beer law, often referred to as Beer's law, which is expressed as:

A = log(I0/I) = abc (1)

where A is the absorbance, I0 is the background intensity, I is the intensity measured with the sample present, a is the absorptivity, b is the sample thickness, and c is the sample concentration.